By Saul Rich (auth.)

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Extra resources for Developments in Industrial Microbiology: Volume 1 Proceedings of the Sixteenth General Meeting of the Society for Industrial Microbiology Held at State College, Pennsylvania, August 30–September 3, 1959

Sample text

Algae can be grown containing as little as 5% nitrogen without appreciable loss in their photosynthetic efficiency (Songers, 1956). The algae should also contain an amino acidcontentwhichis qualitatively and quantitatively sufficient for adequate nutrition of a man for fairly long periods. Our next consideration for selection is that nutritional and pH requirements of the culture be compatible with the system and that concentration ranges over which nutrients can vary be rather wide. If the algae require a very high pH for growth, muchofthe C~ in the system might be tied up in the culture.

Coli designated as E. coli Bfr also had an increased resistance to the presence of hydrogen peroxide in the culture medium. Work has been conducted at our laboratories in an effort to combine and extend these observations. The cultural and exposure conditions used varied somewhat from those of Gaden and Henley (1953). E. 0, and sampies were removed for later use in plate counts. The suspended cells were then irradiated with Co60 at a dose rate of approximately 26,000 r/hr for 4 hr. 4. ~ ••e remaining irradiated cells, constituting the bulk of the irradiated sampie, were then centrifuged down, resuspended in fresh nutrient broth and incubated at 37 C for approximately 48 hr.

Change in surviving fraction of S. marcescens following repeated exposures to gamma radiation. obtained which shows a gradual increase in the radioresistance of the culture. Before general conclusions are drawn concerning the readiness with which radiation-resistant strains may be obtained it should be noted that Gaden and Henley (1953) found a decrease in radiation resistance in E. coli strain 15 after. five successive irradiations. These results may be applicable in pointing out certain of the problems to be faced in space rnicrobiology.

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