By Vandergriff, K.U.; (US); Oak Ridge National Laboratory.; United States. Dept. of Energy. Office of Scientific and Technical Information

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The reference pressure, memorized during a constant flow period of the pump, serves to adjust the cam rotation speed, in a constant pressure mode, during the piston change-over period. If the eluent composition is continuously changing, then a complex algorithm is used to calculate the correction to be brought to the cam rotation speed to make-up for possible deviations from the selected flow during the few past piston cycles. 4 Triple Head Mechanical Reciprocating Pumps When three heads with identical sinusoidal piston movement are combined together with a 120° phase shift between each, the resulting total flow output is periodic.

The ease of operation and reliability of the detector are less easy to quantitize but they are very important practical factors. Ideally, each property of a detector, and especially the range of linearity, the response factor and the detection limits should be determined directly, by pumping solutions of known concentrations through the detector cell. The influence of the various parameters can then be studied easily. Determinations made by injecting known amounts of solutes in a chromatograph are easier to carry out, but they depend on both the detector and the column properties and although easier to carry out, are more difficult to account for.

Extremely low detection limits are claimed (in the 1 x 10- 9 to 1 X 10- 8 range). These performances will be useful only if the detector is properly integrated in a very carefully controlled chromatographic system. Whereas a 10 ppm sensitivity Liquid Chromatographic Equipment 37 is rather easily achieved, further improvement requires higher and higher control of cell temperature and pressure and of mobile phase composition. The entire chromatograph, including the mobile phase tank must be temperature controlled within 1 x 10- 2 dc.

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